Acute post-manipulation pancreatitis after interventions on the major duodenal papilla (experimental study)

Abstract

Objective. To study the morphological consequences of severe acute post-manipulation pancreatitis (APP), the morphological features of its course and the result of the compound "L-17" exposure.
 Materials and methods. Rats weighing 250–300 g were used. Forty operations were performed to create a model of APP on rats. The control group included 20 rats with APP without medication, the experimental group – 20 rats with AKI against the background of "L-17" introduced in the dose of 40 mg/kg once per day.
 Results. In all cases of application of this APP model in rats, the development of acute pancreatitis was noted. The conducted morphological studies showed that APP in rats in the experiment met the criteria for a severe course of acute pancreatitis with the development of purulent peritonitis and necrotizing parapancreatitis with morphological manifestations of systemic inflammation in all the organs studied (with a lethality of 70 %). The use of a new "L-17" in APP in experimental rats showed a decrease in the risk for developing both purulent peritonitis and purulent-necrotic parapancreatitis as well as a 2-fold decrease in mortality (from 70 % to 30 %). A new effect of "L-17" was revealed, i.e. prevention of necrosis of Langerhans islets. The study of the cellular composition of the inflammatory infiltrate showed a decrease in the activity of inflammatory process.
 Conclusions. The efficacy of a new rat model of APP, ranked by severity, was shown. "L-17" alleviates the severity of APP, reducing the risk for developing various purulent complications and systemic inflammation.