ACTIVITY OF STEROID-CONJUGATING ENZYMES IN HATCHLING RED-EARED SLIDER TURTLES EXPOSED TO BISPHENOL-A

Abstract

Event Abstract Back to Event ACTIVITY OF STEROID-CONJUGATING ENZYMES IN HATCHLING RED-EARED SLIDER TURTLES EXPOSED TO BISPHENOL-A Sandrine G. Clairardin1, Ryan T. Paitz1 and Rachel M. Bowden1* 1 Illinois State University, United States Endocrine disrupting chemicals (EDCs) are a class of compounds that impact the functioning of the endocrine system. To more fully understand how EDCs affect an organism, it is important to study not only the endpoint effects of a compound, but also the transient physiological response of the organism to exposure such as the upregulation of sulfotransferases (SULT) and glucuronyltransferases (UGT), two superfamilies of hepatic enzymes. In vertebrates, SULT and UGT are the two main families of enzymes involved in phase II steroid metabolism and these same enzymes are also utilized by the body for clearance and removal of xenobiotics such as EDCs. This study aims to understand which detoxifying pathway is more prominent in EDC metabolism and whether or not the activity of these enzymes is affected by exposure to an EDC. To investigate this aim, red-eared slider (Trachemys scripta) hatchlings were injected with either Bisphenol-A or a vehicle control and livers were harvested 12, 24, or 48 hours post-injection. Liver homogenates (S9 fraction) were used for enzyme assays of SULT and UGT to quantify and compare enzyme activity. Preliminary analyses show a trend for increased enzyme activity of both SULT and UGT by 24 hours post-injection, however there appears to be a great deal of individual variation in ability to respond to exposure through increased enzyme activity. Investigating changes in enzyme activity will provide insight into mechanisms of endocrine disruption, allowing for a more complete understanding of potential threats of endocrine disruptors on humans and wildlife. (Supported by EPA STAR fellowship F09G11129 to S.G.C.) Keywords: Bisphenol-A, Estradiol, steroid conjugation, turtle Conference: NASCE 2011: The inaugural meeting of the North American Society for Comparative Endocrinology, Ann Arbor, United States, 13 Jul - 16 Jul, 2011. Presentation Type: Poster Topic: Endocrine disruption Citation: Clairardin SG, Paitz RT and Bowden RM (2011). ACTIVITY OF STEROID-CONJUGATING ENZYMES IN HATCHLING RED-EARED SLIDER TURTLES EXPOSED TO BISPHENOL-A. Front. Endocrinol. Conference Abstract: NASCE 2011: The inaugural meeting of the North American Society for Comparative Endocrinology. doi: 10.3389/conf.fendo.2011.04.00118 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 21 Jul 2011; Published Online: 09 Aug 2011. * Correspondence: Dr. Rachel M Bowden, Illinois State University, Normal, United States, rmbowde@ilstu.edu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Sandrine G Clairardin Ryan T Paitz Rachel M Bowden Google Sandrine G Clairardin Ryan T Paitz Rachel M Bowden Google Scholar Sandrine G Clairardin Ryan T Paitz Rachel M Bowden PubMed Sandrine G Clairardin Ryan T Paitz Rachel M Bowden Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.