Activities of liver mixed function oxidase system in rats fed trans fat.

Abstract

Rats were fed diets containing either camellia oil or partially hydrogenated corn oil as a source of cis or trans octadecenoate, respectively, in the presence of adequate linoleic acid. After 35 days of feeding the diets, activities of several hepatic drug metabolizing enzymes as well as the content of hepatic microsomal cytochrome P-450 were determined. Geometrical difference in the dietary fat did not affect the amount of microsomal protein nor the content of cytochrome P-450. Also, activities of NADPH cytochrome C reductase, aminopyrine N-demethylase and biphenyl hydroxylase were approximately the same between two groups of rats. Aniline hydroxylase was slightly elevated in the rats fed trans fat. It was concluded that the difference in the geometry of dietary fatty acids had little effect in modulating the hepatic mixed function oxidase system.