Activities of hepatic and extrahepatic microsomal mixed function oxidase enzymes in diabetic and gonadectomized-diabetic rats

Abstract

1. 1. Streptozotocin (STZ)-induced diabetes results in a decrease in hepatic aryl hydrocarbon hydroxylase (AHH) activity in male rats and an increase in female rats, while hepatic 7-ethoxycoumarin 0-deethylase (ECD) activity is increased in both sexes. 2. 2. In castrated male rats, induction of the diabetic state resulted in a further decrease in hepatic AHH activity. In diabetic female rats, ovariectomy did not alter hepatic AHH or ECD activities. 3. 3. STZ-induced diabetes produced an increase in hepatic cytochrome P-450 content. The diabetic state failed to produce an increase in hepatic cytochrome P-450 content of castrated male animals, and only a small increase in castrated female rats. 4. 4. AHH and ECD activities are decreased in lungs of STZ-induced diabetic male and female rats. In diabetic-castrated rats a further decrease in pulmonary ECD activity occurs in both sexes, and in the AHH activity of female rats. 5. 5. The induction of diabetes with STZ increased intestinal AHH and ECD activities in both sexes. In diabetic-castrated rats, intestinal ECD activities were identical to the activities in diabetic male and female animals, while intestinal AHH activities were lower than the activities in diabetic animals but still significantly higher than in control animals of both sexes.