Actividad antifúngica in vitro del extracto crudo diluido del veneno de escorpión Hadruroides charcasus (Karsch 1879) frente a Candida albicans

Abstract

The objective was to evaluate the in vitro antifungal activity of the diluted crude extract of the scorpion venom Hadruroides charcasus (Karsch 1879) against Candida albicans. It was an experimental study, 54 experimental units were used (9 poison concentrations, 2 strains and three repetitions). The venom of the scorpion was obtained by electrical stimulation. The double serial dilution method was determined at different concentrations of 100%, 50%, 25%, 12.5%, 6.25%, 3.125%, 1.56%, 0.78%, 0.39%. To evaluate the antifungal activity, the microdilution method was used to determine the Minimum Inhibitory Concentration (MIC). The results showed that by means of the microdilution method that for the strain n ° 1 presented a minimum inhibitory concentration (MIC) to 25% of the diluted raw venom, very different was for the strain n ° 2 did not present CMI. It was concluded that the findings of the current study show that the diluted crude extract of the scorpion venom H. charcasus possesses antifungal activity on strains of C. albicans, being possible to use it as a template for the design and development of new antifungal drugs.