Antifungal and antioxidant effect of the lachnophyllum ester, isolated from the essential oil of Baccharis trinervis (Lam.) Pers., against dermatophytes fungi

Antônio Carlos Nogueira Sobrinho,Selene Maia De Morais,Raquel Oliveira Dos Santos Fontenelle,Elnatan Bezerra De Souza

doi:10.1590/s1519-99402122542021

Antônio Carlos Nogueira Sobrinho, Selene Maia De Morais + Show 2 more

Open Access

https://doi.org/10.1590/s1519-99402122542021

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Abstract

ABSTRACT Dermatophytes are hyaline fungi that parasitize the keratinized tissue of humans and animals causing mycotic infections. Natural products are promising molecules for the development of new antifungal drugs, due to the phenomenon of resistance and toxicity. This study reports the isolation and identification of lachnophyllum ester and evaluates its antioxidant, antifungal and modulatory activities against dermatophytes fungi. Lachnophyllum ester was obtained using a silica gel column chromatography of the essential oil from the aerial parts of Baccharis trinervis and analyzed by gas chromatography/mass spectrometry. Antimicrobial activity was determined by the broth microdilution method. The modulatory activity assays were performed by the checkerboard technique using lachnophyllum ester and ketoconazole as standard. The lachnophyllum ester exhibited good antioxidant activity as measured by a β-carotene/linoleic acid bleaching system, with 71.43% ± 0.01% inhibition rate. In addition, it showed antifungal activity against Trichophyton rubrum and Microsporum canis strains. In the modulatory assay, interaction between lachnophyllum ester and ketoconazole was synergistic, reducing the minimum inhibitory concentration (MIC) values of the antifungal drug and modulating its antifungal action against dermatophyte strains. In conclusion, lachnophyllum ester has been shown to act as a natural antioxidant compound, as well as an antimicrobial alternative against dermatophyte fungi of the genus Trichophyton and Microsporum.

Highlights

Natural products derived from medicinal plants are of great interest in medicine, in the food industry and, more recently, in biotechnology, through prospecting studies with the purpose of identifying bioactive molecules (Harvey, EdradaEbel and Quinn, 2015)
Lachnophyllum ester, an important compound found in the Asteraceae family, used as chemotaxonomic marker, is a poly-unsaturated aliphatic methyl ester that was first isolated from the plant Lachnophyllum gossypinum Bunge in 1935 (Bruun et al, 1950)
B. trinervis essential oil was submitted to silica gel column chromatography, and the lachnophyllum ester was isolated and characterized by gas chromatography / mass spectrometry, with the data analyzed and corroborated by literature values (Albuquerque et al, 2004)

Summary

Introduction

Natural products derived from medicinal plants are of great interest in medicine, in the food industry and, more recently, in biotechnology, through prospecting studies with the purpose of identifying bioactive molecules (Harvey, EdradaEbel and Quinn, 2015). Various herbal medicines rich in essential oils exhibit antifungal action without side effects to human and animal health This factor is important because many of the current antifungal drugs can cause adverse effects in patients, besides leading to selection for resistance through the adaptation of antifungalresistant microorganisms (Tabassum and Vidyasagar, 2013). Natural products with antioxidant and antimicrobial properties that can stabilize free radical molecules show promise for the development of new phytotherapeutic drugs (Jummes et al, 2020)

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