Abstract
The research on extraction bioactive compound of cocoa pod husk waste and antifungal activity assay by in vitro has been done. The aim of research was to get a phytochemical components extracted from the rind of cocoa, phenolic content and test the bioactivity of extract cocoa pod husk against on pathogenic fungus Fusarium oxysporum. Pod husks used are dried. Samples were extracted by maceration with acetone-water (7: 3) and 70% ethanol. Each extracts were then analyzed using Gas Chromatography-Mass Spectrometry (GC-MS) to determine the active compounds found in the extract cocoa pod husk. The results obtained by GC-MS analysis showed three major components of the compound in ethanol ie: Methyl-9,12-Dienoate Octadeca; 9-octadecenoic acid (Z) -, Methyl Ester; Hexadecanoic acid, 15-methyl, Methyl Ester. Whereas in acetone solvent compounds showed 4 major components, namely: Isopropyl myristate; 1,2-Benzenedicarboxylic Acid, Dioctyl Ester; 9-octadecenoic acid (Z) -, Methyl Ester; Octadecanoic Acid, Methyl Ester. Acetone extract phenolic levels of 94.92 mg GAE / g extract and ethanol extract of 49.92 GAE / g extract. Antifungal testing by the agar diffusion method showed of extract cocoa pod husk with acetone able to inhibit the growth of the fungus Fusarium oxysporum (fungus pathogen on tomatoes), meaning that the extract has the potential to be developed as natural fungicides to suppress the growth of pathogenic fungi.
Highlights
Cacao (Theobroma cacao L.) is one of the country's export commodities Indonesia with high selling value
Based on the description above, it is necessary to investigate phytochemical components extracted from pod husks and know the phenolic content of each solvent used and its potential as a natural fungicide
Materials used to test the antifungal activity of phenolic and testing is gallic acid, Folin-Ciocalteu reagent, paper disc, Potato Dextrose Agar (PDA) and isolates of Fusarium oxysporum
Summary
Cacao (Theobroma cacao L.) is one of the country's export commodities Indonesia with high selling value. The cocoa fruit skin has the potential to be used as an alternative controller to inhibit the growth of microbial pathogens that cause plant diseases. F.oxysporum fungus is the cause wilt and stem rot disease on different types of crops, horticulture and gardening The host of this pathogen are vegetables, onions, potatoes, tomatoes, cabbage, radish, chinese cabbage, mustard-finding meeting, watermelon, melon, papaya, bark, chrysanthemum, orchid, green beans, peppers, cucumber, guava and ginger. That the necessary studies on cocoa pod husk as natural fungicide to control fusarium wilt caused by F. oxysporum. Based on the description above, it is necessary to investigate phytochemical components extracted from pod husks and know the phenolic content of each solvent used and its potential as a natural fungicide
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