Activation of osmotically-activated potassium transporters after injection of mRNA from A6 cells in Xenopus oocytes

Abstract

The different potassium pathways, under iso-osmotic or hypo-osmotic conditions, were examined in Xenopus oocytes that were micro-injected with mRNA from A6 cells. Hypo-osmotically stimulated 86Rb (K +) effluxes could be measured from intact oocytes 1–4 days after injection of 25 ng of poly (A) + RNA isolated from A6 cells. 86Rb (K +) effluxes were 2.2 times higher from oocytes micro-injected with 25 ng of poly(A) + RNA, than from water injected control oocytes. Water-injected oocytes themselves, however, were 7-fold more responsive to a hypo-osmotic shock than non-injected Xenopus oocytes. There was no significant effect of the different K + transport blockers tested (TEA, bumetanide, glybenclamide or quinidine) on the endogenous 86Rb (K +) effluxes from non-injected oocytes in either iso- or hypo-osmotic media. The 86Rb (K +) effluxes from water-and mRNA-injected oocytes in hypo-osmotic media were both inhibited by TEA. In mRNA-injected oocytes the increase in 86Rb (K +) transport following a medium dilution was also inhibited in the presence of glybenclamide or bumetanide. The present study reports that the activation of hypo-osmotically-activated potassium transporters in the oocytes of Xenopus laevis, after injection of mRNA from A6 cells differs quantitatively and in part qualitatively (glybenclamide-sensitivity) from the endogenous K + pathways of non-injected and of water-injected Xenopus oocytes.