Abstract
Bokashi is a type of organic fermented product formed by fungi and bacteria isolated from forests and which coexist synergistically in a liquid fermentative medium enriched with a sugar source. Among these microorganisms, some species of actinobacteria may have biotechnological potential to be used in agriculture. In this sense, the present work aimed to isolate and identify actinobacteria with agricultural biotechnological potential present in fermented organic fertilizer (Bokashi). Bokashi was produced by capturing microorganisms present in the forest soil. Actinobacteria were isolated through successive dilutions of Bokashi samples and grown in culture media Czapeck Modified (MC), Arginine Vitamin Agar (AY*), Inorganic Salt – starch Agar (ISP-4) containing antifungal, and incubated at 30° C for 21 days. Micromorphological analysis and identification was performed using the microculture on slides technique. In all, 22 strains of actinobacteria were isolated and identified, of which 77.27% were from the AY* medium; 13.64% of ISP-4 medium and 9.1% of MC medium. The presumptive identification of the genders showed that the strains were distributed between: 45.45% Nocardia; 36.36% Streptomyces; 9.1% Terrabacter; 4.54% Micromonospora; and 4.54% Mycobacterium.
Highlights
SANTOS, Leonardo da Silva; SOUZA, Giselle Silva de; SILVA, Luana Gomes da; ARAÚJO, Lyslem Riquelem de; SILVA, Mirca Melo Rodrigues da; LOPES, Esmeralda Aparecida Porto
Estudos têm buscado aumentar a produtividade das culturas com a utilização de bioinoculantes (KUMAR; GOPAL, 2015) os quais, na sua maioria, são produzidos com estirpes microbianas fermentadas ou cultivadas separadamente gerando o produto final com inóculos puros (CALVO; NELSON; KLOEPPER, 2014)
Com micélio aéreo ou AC-AL-02 ramificado sob ou sobre o substrato com AC-AL-04 esporos e cadeias em espiarias, podendo
Summary
Volume 6, Número 4 (out./dez. 2021) pp: 3866-3881. https://periodicos.ifal.edu.br/diversitas_journal/ © Diversitas Journal. Actinobactérias com potencial biotecnológico agrícola isoladas de adubo orgânico fermentado (Bokashi). RESUMO: O Bokashi é um tipo de fermentado orgânico formado por fungos e bactérias isolados de matas e que coexistem sinergicamente em meio líquido fermentativo. Algumas espécies de actinobactérias apresentam potencial biotecnológico aplicado na produção de produtos naturais úteis para a agricultura. O presente trabalho teve como objetivo isolar e identificar actinobactérias com potencial biotecnológico agrícola presentes em adubo orgânico fermentado (Bokashi). O Bokashi foi produzido através da captura de microrganismos presentes no solo de mata. As actinobactérias foram isoladas através de diluições sucessivas de amostras do Bokashi e crescidas nos meios de cultura Czapeck Modificado (MC), Arginina Vitamina Ágar (AY*), Inorganic Salt – starch Ágar (ISP-4) contendo antifúngico e incubadas a 30 °C por 21 dias.
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