Abstract
The present report describes an initial characterization of actin from non-infected roots and symbiotic nodules of Phaseolus vulgaris L. cv. Negro damapa. Using anti-actin monoclonal antibodies, a 42-kDa polypeptide was identified in plant extracts. After two-dimensional SDS-PAGE analysis and Western blotting of actin fractions enriched using diethylaminoethyl-resin, the presence of one major isoform of actin in symbiotic nodules and two main isoforms in non-infected roots was revealed. Possible implications of this finding are discussed.
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