Abstract
The pattern of actin synthesis during Drosophila melanogaster embryogenesis was examined by following the appearance of actin specific transcripts. Total or poly(A) +RNA from six embryonic stages was separated on methylmercury gels, transferred to diazobenzyloxymethyl (DBM) paper, and hybridized with actin coding and adjacent sequence probes. The results indicate that a 2-kilobase (kb) transcript is present throughout all embryonic stages; however, a second transcript of 1.77 kb is only detected at an appreciable level by 12 hr of development. These transcripts vary relative to each other in the amount and timing of synthesis, thereby indicating that they are independently regulated.
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