Abstract
Publisher Summary This chapter discusses the isolation of specific ribonucleic acids (RNAs) using deoxyribonucleic acid (DNA) covalently linked to diazobenzyloxymethyl (DBM)-cellulose or DBM paper. Substantial amounts of DNA can be linked covalently to the finely divided DBM cellulose or DBM paper. Either form of insoluble DNA can then be used to select specific species of RNA by hybridization. If RNA is radioactive, DNA paper can be used to analyze the fraction of RNA complementary to the insoluble DNA in a procedure very similar to the one originally developed by Gillespie and Spiegelman. Either DNA-cellulose or DNA paper can be used preparatively to select intact, specific RNAs that can be used subsequently for translation in vitro as a template for reverse transcriptase, as a source of pure infectious RNA, or for any other experiment in which pure, intact RNA is required. The chapter describes the purification of intact RNAs and discusses a quantitative analysis of specific labeled RNAs using DNA paper.
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