Abstract
The stimulation of steroidogenesis in rat adrenal tissue by ACTH, adenosine-3’,5’- monophosphate (cyclic AMP), and N6,O2’;-dibutyryl- adenosine-3’,5’-monophosphate (dibutyryl cyclic AMP) was followed in a superfusion system combined with the continuous fluorometric measurement of corticosterone. Responses to ACTH and to dibutyryl cyclic AMP were dependent upon the total dose of the stimulant and not upon the concentration in the medium. In contrast, the response to cyclic AMP was dependent upon the concentration. The tissue responded to both ACTH and cyclic AMP by an increased rate of production of corticosterone that reached a maximum in 20 min and remained at the maximum until the removal of the stimulus. In contrast, stimulation by dibutyryl cyclic AMP reached a maximum more slowly. The data suggest that the stimulation of adrenal steroidogenesis by ACTH and cyclic AMP involves a rate-limiting step that requires 20 min to reach a steady state. In the case of dibutyryl cyclic AMP, the nucleotide may be ...
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call