Abstract
Phenol is a hazardous chemical known to be widely distributed in aquatic environments. Biodegradation is an attractive option for removal of phenol from water sources. Acinetobacter sp. DW-1 isolated from drinking water biofilters can use phenol as a sole carbon and energy source. In this study, we found that Immobilized Acinetobacter sp. DW-1cells were effective in biodegradation of phenol. In addition, we performed proteome and transcriptome analysis of Acinetobacter sp. DW-1 during phenol biodegradation. The results showed that Acinetobacter sp. DW-1 degrades phenol mainly by the ortho pathway because of the induction of phenol hydroxylase, catechol-1,2-dioxygenase. Furthermore, some novel candidate proteins (OsmC-like family protein, MetA-pathway of phenol degradation family protein, fimbrial protein and coenzyme F390 synthetase) and transcriptional regulators (GntR/LuxR/CRP/FNR/TetR/Fis family transcriptional regulator) were successfully identified to be potentially involved in phenol biodegradation. In particular, MetA-pathway of phenol degradation family protein and fimbrial protein showed a strong positive correlation with phenol biodegradation, and Fis family transcriptional regulator is likely to exert its effect as activators of gene expression. This study provides valuable clues for identifying global proteins and genes involved in phenol biodegradation and provides a fundamental platform for further studies to reveal the phenol degradation mechanism of Acinetobacter sp.
Highlights
Phenol is a toxic compound that is widely distributed in nature, especially in aquatic environments
According to previous experiment (Supplementary methods 1.2), we found that immobilized cells of Acinetobacter sp
The results were in agreement with those obtained by measuring the Adennosine tri-phosphate (ATP) concentration, which demonstrated that the highest biological activity was obtained for Acinetobacter sp
Summary
Phenol is a toxic compound that is widely distributed in nature, especially in aquatic environments. DW-1 on carrier materials, the controllability of this strain, and to find novel candidate proteins or related genes so as to further reveal phenol degradation mechanism, to evaluate the potential for its practical use in bioremediation applications. These aims were achieved using drug sensitivity test and by comparing the proteome and transcriptome of strains grown on different carbon sources and analyzing the expression of major metabolic enzymes during the phenol degradation process. DW-1, lays the foundation for elucidating the mechanism of phenol degradation, and enables future improvements in phenol biodegradation
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