Abstract

Leaf sections of Lolium temulentum showed increased acid invertase activity when incubated in the dark after excision. This activity was principally in the particulate fraction even in extracts from young leaves where particulate activity was initially almost undetectable. Evidence is presented that this activity is not an artefact of extraction. Treatment of the sections with the protein synthesis inhibitor 2-(4-methyl-2,6-dinitroanilino)-N-methyl- propionamide (MDMP) had little effect on the appearance of particulate activity in sections harvested from mature leaves but markedly reduced the appearance of activity in sections from young leaves. The results are discussed in relation to the role of invertase in leaf senescence.

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