Abstract
Recently, Achyranthis radix extract has been studied as a therapeutic agent for dry eye disease that occurs from fine dust. The aim of this study was the development of Achyranthis radix extract-loaded eye drop formulations using lubricants, generally used for artificial tear eye drops. Ecdysterone was used as a marker compound for Achyranthis radix extract and 1% Achyranthis radix extract solution contained 14.37 ± 0.04 μg/mL of ecdysterone. Before formulation studies, a new method was performed to evaluate pigmentation, which might be caused by eye drops of herbal extract. A comparative study of the water retention ability of each formulation and ability to prevent the death of conjunctival epithelial cells in dry conditions was conducted. Moreover, treatment of Achyranthis radix extract (USL) eye drop formulation exhibited a significant inhibitory effect on inflammation in a concentration-dependent manner. The long-term and accelerated stability tests showed that lubricants could contribute to the stability of herbal extracts in solution. In conclusion, hyaluronic acid showed a good effect on the development of eye drop formulation using Achyranthis radix extracts for treating dry eye disease.
Highlights
Dry eye disease (DED) is the condition of having dry eyes, in which the eye surface is damaged due to lack of tears, excessive evaporation of tears, or irregularity of tear component [1]
Ecdysterone exhibited a retention time of 12.8 min (Figure S2A). This peak was confirmed in the USL solution and USL eye drop formulation, F1 (Figure S2B,C)
The lubricants used in this study anti-inflammatory effects in conjunctival epithelial cells
Summary
Dry eye disease (DED) is the condition of having dry eyes, in which the eye surface is damaged due to lack of tears, excessive evaporation of tears, or irregularity of tear component [1]. DED can cause severe pain and limit daily activities such as reading books or using the computer for a long time [3]. These detrimental effects can lead to psychological complications and reduce the quality of life for DED patients [4]. Pharmaceutics 2020, 12, x FOR PEER REVIEW computer for a long time [3]. These detrimental effects can lead to psychological complications and reduce the quality
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
