Acetyl glyceryl ether phosphorylcholine (platelet-activating factor) mediates heightened metabolic activity in macrophages: Studies on PGE, TXB 2 and O −2 production, spreading, and the influence of calmodulin-inhibitor W-7

Abstract

The phospholipid mediator AGEPC (acetyl glyceryl ether phosphorylcholine) was examined for its effects on guinea pig peritoneal macrophages. At a concentration of 10 −9 -10 −6 M, AGEPC evoked release of prostaglandin E (PGE) and thromboxane B 2 (TXB 2) from albumin-elicited macrophages. It also triggerd generation of O − 2 by Corynebacterium parvum-induced cells. Moreover, it caused augmented spreading of macrophages. The calmodulin antagonis W-7 attenuated AGEPC-mediated O − 2 production and cell spreading whereas prostanoid synthesis was enhanced. These novel actions of AGEPC on the major cellular component of the inflammatory process attest to its role as a potent mediator of immunoinflammatory responses.