Abstract
Abstract Studies to evaluate the acetylene (C2H2) blockage method for assessment of denitrifying activity in soil showed that the gas emerging from cylinders of purified C2H2 available commercially contains substantial amounts of acetone and that this impurity is readily utilized by denitrifying microorganisms for reduction of nitrate. They also showed that C2H2 enhanced denitrification of nitrate in soils even when it was purified to remove acetone, but that the effect of acetone‐free C2H2 on denitrifying activity was much smaller than that of acetone‐contaminated C2H2. These observations indicate that use of C2H2 not purified to remove acetone may lead to serious overestimation of denitrifying activity in soils by the C2H2 blockage method.
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