Accurate rotation of ultra-thin glass chamber for single-cell multidirectional observation

Abstract

We report an on-chip single-cell multidirectional observation method based on accurately controlling the ultra-thin glass chamber rotation angles over a microscope. This technique enables one to establish multidirectional visual access to a target cell captured inside of the thin glass chamber. The ultra-thin glass chamber has the advantages of being highly transparent, compact, thin-walled, and less invasive and having an all-closed structure, which efficiently reduces the risk of contamination during observation. The fabricated thin glass chambers’ diameters varied from 1.5 to 3.5 mm which were formed on 200 μm glass slides.