MEMS devices for detecting correspondence between mechanical rotation and ATP consumption in a single biomolecular motor

Abstract

The device for controlling rotational velocity and measuring ATP consumption, simultaneously, of a single F1-ATPase, a rotary biomolecular motor, was developed. This device is equipped with the temperature control device on a slide glass and PDMS micro chamber which volume is on the order of 10 femtoliters. The rotation velocity of a single F1 is controlled by changing the temperature within short response time, 2 seconds. The size of the micro chamber is so small that concentration change of products of a single molecular enzyme can be measured in tens of minutes. To show the feasibility of micro chamber in measuring ATP consumption of a single F1, the catalytic activity measurement of a single HRP enzyme was demonstrated.