Accuracy and repeatability of Roentgen stereophotogrammetric analysis (RSA) for measuring knee laxity in longitudinal studies

Abstract

Roentgen stereophotogrammetric analysis (RSA) can be used to assess temporal changes in anterior–posterior (A–P) knee laxity. However, the accuracy and precision of RSA is dependent on many factors and should be independently evaluated for a particular application. The objective of this study was to evaluate the use of RSA for measuring A–P knee laxity. The specific aims were to assess the variation or “noise” inherent to RSA, to determine the reproducibility of RSA for repeated A–P laxity testing, and to assess the accuracy of these measurements. Two experiments were performed. The first experiment utilized three rigid models of the tibiofemoral joint to assess the noise and to compare digitization errors of two independent examiners. No differences were found in the kinematic outputs of the RSA due to examiner, repeated trials, or the model used. In a second experiment, A–P laxity values between the A–P shear load limits of ±60 N of five cadaver goat knees were measured to assess the error associated with repeated testing. The RSA laxity values were also compared to those obtained from a custom designed linkage system. The mean A–P laxity values with the knee 30°, 60°, and 90° of flexion for the ACL-intact goat knee (±95% confidence interval) were 0.8 (±0.25), 0.9 (±0.29), and 0.4 (±0.22) mm, respectively. In the ACL-deficient knee, the A–P laxity values increased by an order of magnitude to 8.8 (±1.39), 7.6 (±1.32), and 3.1 (±1.20) mm, respectively. No significant differences were found between the A–P laxity values measured by RSA and the independent measurement technique. A highly significant linear relationship ( r 2=0.83) was also found between these techniques. This study suggests that the RSA method is an accurate and precise means to measure A–P knee laxity for repeated testing over time.