Accumulation of vanadium by tunicate blood cells occurs via a specific anion transport system

Abstract

Tunicates, or sea squirts, are known to sequester vanadium to very high concentrations within specialized blood cells. They selectively accumulate the element from seawater against a 10 6- to 10 7-fold concentration gradient, and store it mainly as V(III). The mechanism for this selective accumulation involves the facilitated diffusion of vanadate across the blood cell plasma membrane followed by intracellular reduction to a non-transportable cation. Evidence for this mechanism was obtained by studying vanadate and [ 48V]vanadate influx into living blood cells (vanadocytes). Influx of [ 48V]vanadate into the cells is a rapid ( t 1 2 = 57 s at 0° C ) process which can be saturated ( K m = 1.4 (±2%) mM). Net vanadate accumulation is equal to isotopic influx, and accumulated vanadate is not released by washing cells with EDTA. Uncouplers of oxidative phosphorylation and glycolytic inhibitors have no effect on the rate of influx. Phosphate competes with vanadate for transport, and is itself taken up by the cell. The similar anions, sulfate and chromate, neither inhibit transport, nor are they taken up by the vanadocyte. Influx is inhibited by those stilbene disulfonate derivatives known to bind specifically to the external transport site of the anion exchange protein in the human erythrocyte membrane. During the influx of vanadate, the electron paramagnetic resonance (EPR) signal of intracellular vanadyl increases, indicating that transported V(V) is reduced upon entering the cell. The EPR signal of the blood cells at room temperature is characteristic of unbound V(IV), in agreement with reports that reduced vanadate is not bound to a protein or other macromolecule in these cells.