Accumulation of tolerogenic cells at the site of granulomatous foreign-body reaction

Abstract

Event Abstract Back to Event Accumulation of tolerogenic cells at the site of granulomatous foreign-body reaction Dragana Vucevic1, 2*, Sasa Vasilijic1, 2, Jelena Djokic1, Biljana Bozic3, Ivana Majstorovic1, Sergej Tomic2, Milijana Vasiljevska1, Biljana Draskovic-Pavlovic1, 2 and Miodrag Colic2 1 Institute for Medical Research Military Medical Academy, Serbia 2 Medical Faculty of the Military Medical Academy, University of Defense, Serbia 3 Faculty of Biology, Institute for Physiology and Biochemistry, Serbia The granulomatous foreign-body reaction (GFBR) is a special type of chronic inflammation characterised by infiltration of inflammatory cells (ICs). Using a model of subcutaneous implantation of polyvinyl sponges in rats, we studied the phenotypic and functional characteristics of these cells, with particular reference to the properties of antigen presenting cells and lymphocytes. ICs were isolated from sponge exudates at days 1-21 after implantation. Dendritic cells (DCs) and lymphocytes were purified using a combination of separation gradients, adherence to plastics, and immunomagnetic sorting. We showed that dominant population of ICs at day 1 and 3 were granulocytes, followed by their decrease thereafter. The number of mononuclear cells (MNCs), macrophages and DCs, progressively increased after day 3, reaching maximal values at day 7. Maximal number of lymphocytes was detected at day 10. In addition, total ICs isolated from day 7 till day 10 exerted significant suppressive activity in co-culture with autologous ConA-stimulated thymocytes and alogeneic lymph node T cells. This finding correlated with the increased level of IL-10 in culture supernatants and the increased proportion MHC class II+ IDO+ DCs and CD3+ CD25+ Foxp3+ lymphocytes at day 7 and day 10, respectively. We did not find any significant difference in the number of these cell populations between regional lymph nodes MNCs and peripheral blood MNCs of experimental animals compared to controls. In conclusion, accumulation of cells with tolerogenic characteristics in GFBR might be relevant for the suppression of inflammation and the prevention of unwanted immune response. Keywords: granulomatous foreign-body reaction, Dendritic Cells, regulatory T cells, Lymph Node, chronic inflammation Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Adaptive Immunity Citation: Vucevic D, Vasilijic S, Djokic J, Bozic B, Majstorovic I, Tomic S, Vasiljevska M, Draskovic-Pavlovic B and Colic M (2013). Accumulation of tolerogenic cells at the site of granulomatous foreign-body reaction. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00029 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 08 Mar 2013; Published Online: 22 Aug 2013. * Correspondence: Prof. Dragana Vucevic, Institute for Medical Research Military Medical Academy, Belgrade, Serbia, draganavucevic@yahoo.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Dragana Vucevic Sasa Vasilijic Jelena Djokic Biljana Bozic Ivana Majstorovic Sergej Tomic Milijana Vasiljevska Biljana Draskovic-Pavlovic Miodrag Colic Google Dragana Vucevic Sasa Vasilijic Jelena Djokic Biljana Bozic Ivana Majstorovic Sergej Tomic Milijana Vasiljevska Biljana Draskovic-Pavlovic Miodrag Colic Google Scholar Dragana Vucevic Sasa Vasilijic Jelena Djokic Biljana Bozic Ivana Majstorovic Sergej Tomic Milijana Vasiljevska Biljana Draskovic-Pavlovic Miodrag Colic PubMed Dragana Vucevic Sasa Vasilijic Jelena Djokic Biljana Bozic Ivana Majstorovic Sergej Tomic Milijana Vasiljevska Biljana Draskovic-Pavlovic Miodrag Colic Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.