Abstract
In a previous paper1 I reported that when living cells of Valonia macrophysa were immersed for periods of 2-3 days in sea water to which had been added isotonic NaCl solution (10-50% by volume) there occurred an increase in the concentration of potassium and a decrease in that of sodium relative to that of chloride. Since these changes were in the direction opposite to those made in the composition of the external medium, it was concluded that this experiment furnished crucial proof that the normal condition in Valonia was not an equilibrium state. The validity of the data has been doubted by Cole on statistical grounds,2 and by Jacques and Osterhout on experimental grounds.3 I have therefore repeated my experiments in the endeavor to meet these criticisms.Experiments were done on Valonia macrophysa at Naples, which tended to confirm my earlier results, but they were unsatisfactory statistically. Recently similar experiments were done on specimens of Valonia ventricosa at Tahiti. These specimens were extr...
Published Version
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