Abstract
Abscisic acid (ABA) is a key plant growth and stress hormone involved in many biological processes. It has been shown to be involved in Reactive Oxygen Species (ROS) generation. Class-III Peroxidases (PODs) are known to maintain oxidative stress induced-ROS at sub-lethal levels in plants under abiotic stress conditions, but, studies documenting how ABA regulates boiling stable class-III PODs are still a matter of conjuncture. In this study, the ABA-induced changes on ROS and ROS scavenging class III boiling stable POD were studied in the embryos of different cultivars of wheat. Simultaneous analysis of ROS contents, activities of ROS-scavenging class- III boiling stable POD enzymes gave an integrative view of physiological state and detoxifying potential under conditions of sensitivity and tolerance. Indices of oxidative stress viz., superoxide radical and H 2 O 2 content increased under ABA treatment in a genotype dependent manner. It was observed that cultivars :PBW 550, HD 2967 and PBW 621 have more efficient mechanism to scavenge ROS species as shown by increase in BsPOD activity accompanied by enhanced expression of boiling stable POD isoenzymes. Based on results it can be inferred that embryos of cvs. PBW 550, HD 2967 and PBW 621 have more capacity to perform biological antioxidative reactions to combat ABA-induced oxidative stress.
Highlights
The phytohormone abscisic acid (ABA) regulated various growth and developmental related processes in plants (Shinozaki & Yamaguchi-Shinozaki, 2007)
ABA-induced changes in fresh weight (FW), dry weight (DW) and Tissue water content (TWC)
This study provided a particular insight into the changes in Reactive Oxygen Species (ROS) production and boiling stable antioxidant POD activities in embryos under ABA-induced oxidative stress
Summary
The phytohormone abscisic acid (ABA) regulated various growth and developmental related processes in plants (Shinozaki & Yamaguchi-Shinozaki, 2007). Several roles have been attributed to plant class-III Prxs in response to biotic and abiotic stresses such as in the modification of cell wall like suberin polymerization, cross-linking of structural non-enzymatic proteins, and ability to cleave cell wall polysaccharides, leaf expansion, fruit growth, germination, nodulation (Cosio & Dunand, 2009). They may have diverse roles possibly due to large number of isoforms (isoenzymes). Some studies indicated that activities of antioxidant enzymes are correlated with plant tolerance to abiotic stress (Ozkur et al, 2009; Wang et al, 2009)
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