Abstract
The hardening-triggered accumulation of a barley cold-regulated protein of 75kDa (COR 75) has been investigated in several genotypes featuring contrasting frost tolerance capacity. COR 75 is accumulated at low temperatures, suggesting its involvement in the development of frost resistance. COR 75 is accumulated faster during the first days at low temperature in frost resistant varieties when compared with extremely sensitive varieties; nevertheless after 7 days of hardening, COR 75 is expressed at the same level in all genotypes tested despite their differences in frost tolerance. Besides low temperature, COR 75 can also be induced by ABA or drought stress indicating that this protein may be a general response to variation in tissue moisture status. Using a polyclonal antibody raised against COR 75, it has been demonstrated that this protein is immunologically related to two wheat cold-regulated proteins of 160 and 200 kDa. The role of COR 75 in the development of hardening is discussed.
Published Version
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