Accessing the anti-microbial activity of cyclic peptide immobilized on reduced graphene oxide

Abstract

Herein, we prepared and characterized two antimicrobial cyclic peptides immobilized on reduced graphene oxide (rGO) and accessed their anti-bacterial towards Gram-negative (Pseudomonas aeruginosa) and Gram-positive (Staphylococcus aureus) bacterias. The covalent interaction between the tyrosine and histidine based cyclic peptides and the rGO occurred via the carboxylic groups of the reduced graphene and the amino side chain groups of the cyclic peptides. A selective antibacterial effect is observed for the cyclic peptides functionalized rGO. The inhibition zone test showed that both the tyrosine and histidine cyclic based peptide immobilized on rGO exhibited higher anti-bacterial potency towards the bacteria in comparison to bare rGO which can be attributed to the synergistic effect of the antimicrobial peptide and rGO.