Abstract
Background and objectives: Skin grafting is a method usually used in reconstructive surgery to accelerate skin regeneration. This method results frequently in unexpected scar formations. We previously showed that cutaneous wound-healing in normal mice is accelerated by a micrograft (MG) technique. Presently, clinical trials have been performed utilizing this technology; however, the driving mechanisms behind the beneficial effects of this approach remain unclear. In the present study, we focused on five major tissue reactions in wound-healing, namely, regeneration, migration, granulation, neovascularization and contraction. Methods: Morphometrical analysis was performed using tissue samples from the dorsal wounds of mice. Granulation tissue formation, neovascularization and epithelial healing were examined. Results: The wound area correlated well with granulation sizes and neovascularization densities in the granulation tissue. Vascular distribution analysis in the granulation tissue indicated that neovessels extended and reached the subepidermal area in the MG group but was only halfway developed in the control group. Moreover, epithelialization with regeneration and migration was augmented by MG. Myofibroblast is a known machinery for wound contraction that uses α-smooth muscle actin filaments. Their distribution in the granulation tissue was primarily found beneath the regenerated epithelium and was significantly progressed in the MG group. Conclusions: These findings indicated that MG accelerated a series of wound-healing reactions and could be useful for treating intractable wounds in clinical situations.
Highlights
Delay in wound-healing prolongs hospital stay and increases medical cost, and decreases the quality of life of patients
Basic fibroblast growth factor is widely adopted for various kinds of wound treatments because it directly stimulates the growth of granulation tissue including fibroblasts and endothelial cells [6]
Skin wound-healing reactions progress with fibroblast proliferation, collagen matrix formation and neovascularization in newly formed granulation tissue, on which epithelialization occurs
Summary
Delay in wound-healing prolongs hospital stay and increases medical cost, and decreases the quality of life of patients. Wound-healing is a fundamental tissue reaction, by which wounds heal after granulation tissue formation and tissue regeneration at the sites of tissue defect. If such reactions do not progress appropriately, especially in patients with metabolic disorders including diabetes, nutritional deficiency and blood flow disturbance, treatment becomes challenging [1,2]. We focused on five major tissue reactions in wound-healing, namely, regeneration, migration, granulation, neovascularization and contraction. Myofibroblast is a known machinery for wound contraction that uses α-smooth muscle actin filaments Their distribution in the granulation tissue was primarily found beneath the regenerated epithelium and was significantly progressed in the MG group. Conclusions: These findings indicated that MG accelerated a series of wound-healing reactions and could be useful for treating intractable wounds in clinical situations
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