Abstract
Objective: The main objective of the research work was to fabricate sacran hydrogel film containing keratinocyte growth factor (Sacran/KGF-HGF), and to evaluate their wound healing ability in alloxan-induced diabetic mice model.Methods: The physicochemical characterization of Sacran/KGF-HGF were investigated by thickness, tensile strength, swelling ratio, x-ray diffractometer (XRD), scanning electron microscope (SEM), and biodegradability. The wound healing ability was investigated by creating two full-thickness excisional wounds inalloxan-induced diabetic mice.Results: The thickness, tensile strength, and swelling ratio results showed that KGF in the Sacran/KGF-HGF improved not only the thickness of sacran hydrogel film (Sacran-HGF), but also the tensile strength and swelling ability of Sacran-HGF. The XRD and SEM results confirmed that the Sacran/KGF-HGF were amorphous and similar morphology to Sacran-HGF, respectively. The biodegradability results revealed that the Sacran/KGF-HGF degraded for about 41.29% in trichloroacetic acid (TCA) and 22.92% in TrypLE™ (recombinant enzyme) solutions. In addition, KGF improved the degradability of Sacran/KGF-HGF in both solutions. Interestingly, the Sacran/KGF-HGF, which was applied on wound site, considerably improved the wound healing ability of Sacran-HGF at 6, 9 and 12 d in alloxan-induced diabetic mice model, compared to control (non-treated).Conclusion: These results suggest that KGF has the potential to promote the chronic wound healing ability of Sacran-HGF.
Highlights
The skin, a layered organ, covers the entire human body with the main function as a protector for preventing skin dehydration and microorganism’s penetration from outside the body
These results suggest that Keratinocyte Growth Factor (KGF) has the potential to promote the chronic wound healing ability of Sacran-hydrogel films (HGFs)
We examined whether Sacran-HGFs can be formed in the presence of KGF for chronic wound therapy, and evaluated their physicochemical properties
Summary
The skin, a layered organ, covers the entire human body with the main function as a protector for preventing skin dehydration and microorganism’s penetration from outside the body. The wound healing process basically consists of several stages, namely hemostasis, inflammation, proliferation, vascularization, production and restoration of the extracellular matrix (ECM)[2]. The healing process in chronic wounds, especially in diabetic condition, will be disrupted with persistent inflammation, slow migration of keratinocytes and fibroblast, abnormal regulation of chemokines and production of growth factor, the irregular response of inflammatory cells, and inhibition of angiogenesis [3,4,5]. Regenerative therapy using growth factors can be used to accelerate the chronic wounds healing [8, 9]. One of the essential growth factors for impaired healing therapy is Keratinocyte Growth Factor (KGF). KGF is a major cellular component of the epidermis, important for the maintenance of the barrier, and for the restoration of the wound through a process known as epithelization [10]. KGF acts on epithelial cells and stimulates cell proliferation and migration [11]
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