Abstract
Methanol or petroleum ether extracts of entire Pinus leucodermis Ant. needles did not depress growth of five aquatic hyphomycetes, whereas methanol extract of needle powder was inhibitory. Extraction with the two solvents attacked surface structures of entire needles. Four needle types (untreated, halved, petroleum ether or methanol extracted) were immersed in a stream for 21 days. After immersion, untreated needles carried significantly lower numbers of conidiophores of aquatic hyphomycetes than any of the three other needle types, and methanol extract made from a powder of untreated needles inhibited fungal growth significantly more than extracts from the other three needle types. Sliced, or preextracted needles apparently lost inhibitors at a faster rate than untreated needles. The content of methanol-soluble phenolics decreased in needles exposed to the stream. This loss was highest in halved needles (72.9%) and lowest in untreated needles (16.6%).
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