Abstract
Previous lncRNA microarray screening found that the AC002454.1 gene was highly expressed in endometriosis (EMS), and these expression levels were highly correlated with cyclin-dependent kinase-6 (CDK6). This study investigated the expression level and correlation between AC002454.1 and CDK6 in endometrium tissues and the influence of these changes in expression upon the biological behavior of eutopic endometrial cells. We confirmed AC002454.1 and CDK6 mRNA and protein were highly expressed in ectopic and eutopic endometrial tissue from patients with EMS and were clearly correlated. In vitro, both AC002454.1 and CDK6 positively regulated the proliferation, migration and invasion ability of eutopic endometrial cells and could promote the transformation of cells from G0/G1 phase to S phase. AC002454.1 and CDK6 may have synergistic effects, thereby affecting the biological behavior of endometrial cells, and thus promote the progression of EMS.
Highlights
Endometriosis (EMS) is an estrogen-dependent disease which is refractory and associated with cell dysfunction
We investigated the effect of AC002454.1 and cyclin-dependent kinase-6 (CDK6) in the biological behavior of eutopic endometrial cells from patients with endometriosis
Using GAPDH as an internal reference, real-time polymerase chain reaction (RT-PCR) indicated that the relative expression of AC002454.1 and CDK6 mRNA in EU were significantly higher than that of normal endometrium tissues (NE) (P < 0.05; Fig. 1A and B) and were positively correlated (Pearson correlation coefficient: 0.691, P < 0.001)
Summary
Endometriosis (EMS) is an estrogen-dependent disease which is refractory and associated with cell dysfunction. The retrograde menstruation phenomenon represents a classic theory relating to the pathogenesis of EMS (Burney & Giudice 2012). Studies have shown that patients with EMS in the lining of the endometrium, especially during the secretory phase, appear different in terms of endometrial cell structure, migration, invasion, proliferation, angiogenesis, apoptotic ability and the expression of certain genes (Sundqvist et al 2012). These studies have provided further evidence for ‘eutopic endometrium determinism’ (Zhang et al 2015)
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