Acı Kavun (Ecballıum Elaterıum L. 1758)’un Sazan (Cyprınus Carpıo L. 1758) Balıkları Kan Hücre Morfolojilerine Etkileri

Abstract

It is well-known that for centuries, humans have used plants and herbal remedies to treat many diseases, in addition to the treatment options brought and applied by modern medicine, which relies on scientific studies and actual evidence. One of these plants is the squirting cucumber (Ecballium elaterium), or exploding cucumber. Despite its therapeutic properties, the squirting cucumber may also cause serious biological damage to humans, as demonstrated by a number of studies. This study was conducted with the aim of determining whether the juice obtained from ripe fruits of squirting cucumber has any effect on the cell morphology of common carp (Cyprinus carpio). Juice application was carried out on 4 groups, composed of 1 control and 3 experimental groups. Squirting cucumber juice was added to three aquariums containing different experimental groups, in three different ppm levels, for 72 hours. Afterwards, blood smear samples of the fish were prepared, and the preparations were scanned under a microscope in order to determine the changes in blood cell morphology. The results demonstrated that squirting cucumber juice caused micronucleus formation during cell division in C. carpio blood cells, and a number of abnormalities in blood cell morphology including Lobed nucleus (LB), Bud nucleus (BL), Notched nucleus (NT) and Binucleated erythrocytes (BN).