Abundant Expression of Guidance and Synaptogenic Molecules in the Injured Spinal Cord

Anne Jacobi,Florence M Bareyre,Anja Schmalz

doi:10.1371/journal.pone.0088449

Anne Jacobi, Florence M Bareyre + Show 1 more

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https://doi.org/10.1371/journal.pone.0088449

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Abstract

BackgroundSpinal interneurons have emerged as crucial targets of supraspinal input during post-injury axonal remodelling. For example, lesioned corticospinal projections use propriospinal neurons as relay stations to form intraspinal detour circuits that circumvent the lesion site and contribute to functional recovery. While a number of the molecules that determine the formation of neuronal circuits in the developing nervous system have been identified, it is much less understood which of these cues are also expressed in the injured spinal cord and can thus guide growing collaterals and initiate synaptogenesis during circuit remodelling.Methodology/Principal FindingsTo address this question we characterized the expression profile of a number of guidance and synaptogenic molecules in the cervical spinal cord of healthy and spinal cord-injured mice by in situ hybridization. To assign the expression of these molecules to distinct populations of interneurons we labeled short and long propriospinal neurons by retrograde tracing and glycinergic neurons using a transgenically expressed fluorescent protein. Interestingly, we found that most of the molecules studied including members of slit-, semaphorin-, synCAM-, neuroligin- and ephrin- families as well as their receptors are also present in the adult CNS. While many of these molecules were abundantly expressed in all interneurons examined, some molecules including slits, semaphorin 7a, synCAM4 and neuroligin 1 showed preferential expression in propriospinal interneurons. Overall the expression pattern of guidance and synaptogenic molecules in the cervical spinal cord appeared to be stable over time and was not substantially altered following a midthoracic spinal cord injury.ConclusionsTaken together, our study indicates that many of the guidance and synaptogenic cues that regulate neuronal circuit formation in development are also present in the adult CNS and therefore likely contribute to the remodelling of axonal connections in the injured spinal cord.

Highlights

For successful wiring of the nervous system axons need to navigate and establish synaptic contacts with their proper target cells
Our results show that members of the slit, semaphorin, synCAM, neuroligin- and ephrinB- families are abundantly expressed in spinal interneurons both before and after spinal cord injury
The process of axon pathfinding is mediated by a number of guidance molecules, among them slits and their receptors, which have been shown to have a repulsive effect on axons during development [22,23] and have been proposed to restrict axonal growth at the lesion site following spinal cord injury [24]

Summary

Introduction

For successful wiring of the nervous system axons need to navigate and establish synaptic contacts with their proper target cells. SynCAMs [7] and neuroligins [8], for example, can act as pre-synaptic organizers while neurexins [9], and ephrinBs [10] are postsynaptic organizers. To what extend these molecules regulate pathfinding and synapse formation of re-growing axons in the damaged adult nervous system is so far only incompletely understood. While a number of the molecules that determine the formation of neuronal circuits in the developing nervous system have been identified, it is much less understood which of these cues are expressed in the injured spinal cord and can guide growing collaterals and initiate synaptogenesis during circuit remodelling

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