Abstract
The concentration of free and protein-bound urate in plasma was studied in control and gouty subjects. The uric acid bound to the plasma proteins was estimated by ultrafiltration method using Amicon's micropartition system. The total concentration of uric acid in the plasma and in the ultrafiltrate was analyzed by an enzymatic colorimetric method and the amount of uric acid binding was obtained from the difference between uric acid in plasma and in ultrafiltrate. In gouty patients the percentage of uric acid is quite the same that in control subjects, but it must be considered in relation to the higher values of uricemia; consequently, what is really increased is the free uric acid amount step by step with the increase of uricemia. So, the whole amount of free uric acid is available for the deposition in the tissue. The reduced binding capacity showed by gouty patients in comparison with healthy subjects is also confirmed by the results following to an hypoxanthine load per os, carried out in the two groups.
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