Abstract WMP68: The MicroRNA Expression Analysis for Thrombus From Acute Ischemic Stroke

Abstract

Background: The thrombus retrieved from mechanical thrombectomy among stroke patients could provide important information regarding stroke mechanism and its prognosis. We investigated microRNA expression pattern from thrombus to differentiate stroke mechanism. Methods: Between September 2016 and March 2019, acute ischemic stroke patients who received intra-arterial mechanical thrombectomy from Chung-Ang University Hospital, Seoul, Korea were eligible to be included. After obtaining informed consent, the thrombus was stored at -70°C nitrogen tank. First, we performed microRNA microarray analysis for the patients with clearly identified stroke mechanisms including large artery atherosclerosis, cardioembolism with atrial fibrillation and cardioembolism with valvular heart disease. Second, those microRNAs derive from microarray analysis were validated by quantitative real time polymerase chain reaction, and correlation analyses were performed between microRNA levels and clinical data. Finally, the expression levels of microRNAs were compared between the patients with early neurological deterioration (END) after stroke and those without. Results: A total of 48 thrombi had been retrieved from 51 patients. The microarray analysis of microRNA revealed that 42 microRNAs were significantly altered among the three groups out of 869 examined microRNAs. Ejection fractions were negatively correlated with miR-450b-5p levels (r=-0.448, p<0.0001) and e/e’ were positively correlated with miR-378f (r=0.640, p<0.001). The patients with END exhibited higher levels of miR-93-5p and lower levels of miR-629-5p. Conclusion: The microRNA expression pattern can provide information regarding stroke mechanisms, underlying heart dysfunction and prognosis after recanalization treatment.