Abstract PR157

Abstract

Background & Objectives: Backgroud Growing evidences indicate that general anesthetics will cause harmful effect on developing brain, resulting in deficiency in cognitive function. While little is about the effect of maternal anesthesia exposure on the learning and memory in offspring. Since there are more than 2% pregnant women have to suffer general anesthesia for invasive medical examination or non-obstetric surgery such as for acute cholecystitis, appendicitis and trauma. Objective The aim of presents study is to study the effect of maternal exposure to general anesthetics on the learning and memory. Materials & Methods: Methods Maternal rats exposed to common used genesthetics in different pregnant stage. On postnatla day 30, the learning and the memory of offspring rats were tested by morris water maze. HDAC inhibitor vorinostat was intraperitoneally injected to the offspring 2h before each morris water maze trial. The mRNA and protein levels of histone deacetylase 2 (HDAC2), N-methyl-D-aspartate (NMDA) receptor subunit NR2B and hippyragranin (HGN) were detected by real time RT - PCR adn immunohistochemistry. The hippocampus neuronal ultrastructure was analyzed by emission electron microscope. Results: Results Our results showed that maternal exposure to midazolam isoflurane or sevoflurane during early gestation has no significant effect on the learnin and memory in morris water maze test. While propofol, ketamine or enflurane exposure during early gestation caused dosage - dependent learning and memory impairment in offspring rats. More over, maternal exposure to isoflurane or sevoflurane during late pregnancy caused significant learning and memory impairment. The mRNA and protein levels of HDAC2 and HGN increased significantly in the hippocampus in learning and meory damaged offsprings, while the mRNA and protein levels of NR2B decreased significantly. Neuronal ultrastructure damage in hippocampus had been observed in these offspring rats. Vorinostat reversed these changes obviously. Conclusion: Maternal exposure to general anethesia will cause learning and memory impairment in offspring dependent on the anesthetic, it’s dosage and the exposure timing. Epigenetics may be involve in the pathogenesis. Disclosure of Interest: None declared