Abstract P1-01-12: A Novel Method for Sentinel Lymph Node Biopsy by Indocyanine Green Fluorescence Technique in Breast Cancer

Abstract

Abstract Background: Sentinel lymph node (SLN) biopsy is the standard method to assess the actual axillary lymph node status in breast cancer. Currently dye techniques, radioisotope techniques or combined techniques are usually used for SLN detection. Recently, near infrared fluorescence imaging has been applied clinically in a breast cancer patient to identify SLN. In this study, the feasibility of SLN biopsy using the indocyanine green (ICG) technique were evaluated. Methods: The study involved four hundreds eleven patients with clinically node negative early breast cancer who underwent SLN in three institutes. A combination of ICG as a fluorescence emitting source and blue dyes were injected in the subareolar area and lymphatic flows were traced with a charge coupled device camera and a real-time image guided surgery enabled to identify the fluorescence image of SLN after meticulous dissection. Results: The subcutaneous lymphatic channels were detected precisely in all cases. The identification rate of SLN was 99%, (408/411) with a mean number of 2.3±1.2 (range, 1-9) nodes identified per patient. Only one SLN harvested in 30.1% of patients, two in 29.4%, three in 23.9% and four or more in 15.9% of patients. Thirty nine cases (9.5%) had SLNs involved and all of them were ICG positive and 30 of 39 patients (77%) had one SLN involved. Conclusions: This ICG fluorescence method is simple and achieves a high SLN identification rate. This technique does not require a facility equipped to use radioisotopes. This means that SLN biopsies could even be performed in a small hospital. Orderly and sequential dissection along the lymphatic flow may provide higher sensitivity compared with the conventional radioisotope method. A direct comparison between the radioisotope and ICG fluorescence methods is now required. Citation Information: Cancer Res 2010;70(24 Suppl):Abstract nr P1-01-12.