Abstract
Abstract Insulin-like growth factor binding proteins (IGFBPs) are components of the IGF signaling system, which is comprised of ligands IGF-I and IGF-II as well as a family of transmembrane receptors including the IGF-IR and IGF-IIR. IGFBPs bind to both IGF-I and IGF-II with high affinity and are essential for transporting IGFs. This prolongs their half-lives and regulates the availability of free IGFs for interaction with IGF receptors, thereby modulating the effects of IGFs on growth and differentiation. Amongst the six IGFBPs, IGFBP-3, the only IGFBP species regulated by the p53 tumor suppressor, is a well-documented potent tumor suppressor in a variety of human cancer. IGFBP-3 has been further suggested as a potent anti-metastatic factor. Recently, more direct evidence for antitumor effect of IGFBP-3 has been reported in various cancer cells including triple negative breast cancer (TNBC) cells in vitro and in vivo, but the underlying mechanisms of antitumor action of IGFBP-3 are largely unknown. We have identified a new class of cell death receptor called IGFBP-3R, which constitutes a novel cell-death receptor that mediates the anticancer effects of IGFBP-3 in human cancer. IGFBP-3 is significantly reduced whereas IGFBP-3R is broadly expressed in many human cancer types such as breast, prostate, lung and colon cancer. In order to discover novel IGFBP-3/IGFBP-3R antitumor signaling as a therapeutic target, a panel of IGFBP-3R specific monoclonal antibodies (mAbs) have been generated and further identified IGFBP-3R agonistic mAbs acting like IGFBP-3 showing anticancer effects in human cancer cells. Further in vivo studies using a bioluminescent orthotopic TNBC mouse model and Patient Derived Xenograft (PDX) TNBC mouse models demonstrated that administration of IGFBP-3R agonistic mAbs results in significant shrinkage of primary and metastatic tumors, accompanying induction of caspase-dependent apoptosis and suppression of tumor-activated NF-κB signaling. More importantly, IGFBP-3R agonistic mAbs have synergistic anticancer effects with therapeutic agents such as carboplatin, cisplatin and Bcl-2 family inhibitors in a variety of human cancer including breast, pancreatic cancer and leukemia, and it was further identified molecular mechanisms involved in its synergy in vitro. These findings clearly demonstrate the IGFBP-3/IGFBP-3R system as a new target and the IGFBP-3R agonists such as IGFBP-3R agonistic mAbs as a new monotherapy and a combinational therapy with therapeutic agents therapy for a variety of human cancer. Citation Format: Youngman Oh, Qing Cai, J. Chuck Harrell, Jennifer Koblinski. A novel targeted antibody therapy for cancer: Exploring the IGFBP-3/IGFBP-3 receptor axis as an antitumor and anti-metastatic signaling in cancer [abstract]. In: Proceedings of the AACR-NCI-EORTC International Conference on Molecular Targets and Cancer Therapeutics; 2019 Oct 26-30; Boston, MA. Philadelphia (PA): AACR; Mol Cancer Ther 2019;18(12 Suppl):Abstract nr B096. doi:10.1158/1535-7163.TARG-19-B096
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