Abstract A4: Antagonistic effects of sulphated polysaccharides from Turbinaria conoides (J. Agardh) on tumor cell migration and angiogenesis

Abstract

Abstract A4 Seaweeds are considered to be a rich source of biologically active substances like antioxidants, anti-viral, anti-inflammatory and anticoagulant agents. There are some evidences that seaweeds contain compounds with a relatively high antioxidant and antiproliferative activity. Seaweeds are low in fats but contain vitamins and bioactive compounds, like terpenoids and sulfated polysaccharides. Sulphated polysaccharides from brown algae are found to exhibit diverse biological activities like anticoagulant, antihyperlipidemic, antiviral, antioxidant, antitumor, antimetastatic and antiangiogenic activities. In this study, the antiproliferative, antiinvasive and antiangiogenic properties of sulphated polysaccharides from brown seaweed, Turbinaria conoides against colon carcinoma cell line COLO 320DM were investigated. Crude sulphated polysaccharide was extracted from T. conoides, collected from Mandapam coast, South east coast of India. The antiproliferative activity of the seaweed polysaccahrides on COLO 320DM was evaluated by MTT assay and DNA fragmentation studies. The inhibitory potential on the migration of this invasive cell line was assessed by wound healing assay. The effect of sulphated polysaccharides against angiogenesis was investigated by chorio allantoic membrane (CAM) assay in chick embryo. It was observed that sulphated polysaccharides from T. conoides inhibited the proliferation of COLO 320DM cells at a concentration of 1mg/ml by 40% at 24 hours. DNA fragmentation as evidenced by ladder formation in agarose gel indicates that the antiproliferative activity of seaweed polysaccharides is due to the induction of apoptosis. The migration of the carcinoma cell line was effectively inhibited by the seaweed extract. The antiinvasive property increased with increasing concentrations. However, the activity was more or less similar at 24 and 48 hours. In the CAM assay, it was found that sulphated polysaccharides from the brown seaweed inhibited vasculogenesis in a developing chick embryo. These results clearly indicate that sulphated polysaccharides from brown algae are potential molecular leads for antimetastatic drug development. Citation Information: Cancer Prev Res 2008;1(7 Suppl):A4.