Abstract
Angiogenesis is a fundamental physiological process with strong implications in tissue homeostasis. Animal models helping to identify how angiogenesis is regulated are fundamental to answer many biological questions. Chick embryo chorioallantoic membrane (CAM) assay is one of the most employed methods to study angiogenesis. In this study we applied a scientometric approach to evaluate the employment of CAM assay in published articles. Temporal trends indicated that CAM assay was the preferred method to investigate angiogenesis over time. The publications had a significant number of citations and the impact factor of journals publishing articles is relevant for the scientific community. A total of 52 different research areas have articles published using this particular technique. Oncology is the research field in which CAM assay was mostly used. Accordingly, tumor-derived cell lines were the most frequent sample tested on CAM. We also identified that 73,6% of articles published used only CAM assay to answer questions concerning angiogenesis. We concluded that although the CAM assay is a classical approach, that does not need so much infrastructure and financial support to be performed, it is a well-accepted technique by the scientific community. In addition, this methodology has gain attention in scientific community because no pain is experienced by the chick and they are minor ethical concerns to employ this method. Moreover, this data can help researchers who are unfamiliar with the CAM assay to identify if this particular method is suitable for their research.
Highlights
Angiogenesis is a complex biological process by which new blood vessels form from preexisting vascular tissue
Our analysis identified a total of 2,248 research articles that used chorioallantoic membrane (CAM) assay to address multiple biological questions
The publications had a significant number of citations (Fig. 1b) and the impact factor of journals publishing articles using this technique is relevant for the scientific community (Fig. 1c, d)
Summary
Angiogenesis is a complex biological process by which new blood vessels form from preexisting vascular tissue. At the most basic level, it involves proliferation, migration and differentiation of endothelial progenitor cells (FOLKMAN, 2003). Extensive efforts have been made to develop therapeutic strategies to promote or to inhibit angiogenesis (FISHER et al, 2006), owing to its several medical applications. A compound that is able to induce angiogenesis can be useful for tissue engineering, boosting cell proliferation and promoting wound healing (ALMEIDA et al, 2014). A compound that inhibits angiogenesis could be used to reduce the abnormal vasculature found in several types of solid tumors (RIBEIRO et al, 2012). Millions of patients worldwide are being treated with compounds that regulate angiogenesis
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
