Abstract 89: The microRNA-3622b-3p contributes to tumor progression and metastasis through an miR-3622-AIFM2 axis in prostate cancer

Abstract

Abstract microRNA (miR)-3622 family is composed of two members, miR-3622a/b, but their roles remain debatable. In the present study, we found that expression of miR-3622a was lower, whereas expression of miR-3622b-3p was higher in human prostate cancer tissues than in normal prostate tissues. miR-3622a-3p inhibited cell migration and invasion of human prostate cancer cells, whereas miR-3622b-3p facilitated cell proliferation, migration, and invasion. To address the opposing roles of miR-3622 family members in various human prostate cancer cell lines, we knocked out endogenous miR-3622, including both miR-3622a/b. Our results showed that miR-3622 knockout reduced cell proliferation, migration, and invasion in vitro and tumor growth and metastasis in vivo. Furthermore, we found that AIFM2, a direct target of miR-3622b-3p, is responsible for miR-3622 knockout-induced apoptosis, identifying an miR-3622-AIFM2 axis that contributes to oncogenic function during tumor progression. The results show that miR-3622b-3p is upregulated in human prostate cancers and has an oncogenic function in tumor progression and metastasis through an miR-3622-AIFM2 axis. Citation Format: Lizhong Wang, Chao Zhang, Ping Ye. The microRNA-3622b-3p contributes to tumor progression and metastasis through an miR-3622-AIFM2 axis in prostate cancer [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2022; 2022 Apr 8-13. Philadelphia (PA): AACR; Cancer Res 2022;82(12_Suppl):Abstract nr 89.