Abstract 739: A disintegrin and metalloproteinase 8 as a potential blood biomarker for early diagnosis of gastric cancer

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Abstract Purpose: We evaluate the clinical significance of A Disintegrin and Metalloproteinase 8 (ADAM 8) as a potential blood biomarker for gastric cancer (GC). Materials & Methods: Blood ADAM 8 was measured by ELISA and CEA, Cytokines/chemokines (IL-23, SDF-1α/CXCL12, IL-8, and sCD40L) were measured by chemiluminescent immunoassay. They were compared among 5 groups; normal/gastritis, high-risk, early GC (EGC), advanced GC (AGC) without distant metastasis and AGC with distant metastasis by one-way analysis of variance in both training (n=80) and validation dataset (n=241). Clinicopathological features of GC and GC-associated cytokines were evaluated for their correlations with blood ADAM 8. To evaluate the diagnostic accuracy to predict GC, receiver operating characteristic (ROC) curve and logistic regression were used. Results: In both training and validation dataset, blood ADAM 8 increased along the GC carcinogenesis (ANOVA, all of p<0.01). Blood ADAM 8 were significantly higher in EGC and AGC compared with high-risk and normal (post-hoc Bonferroni, all of p<0.01). However, there was no significant difference among cancer groups. Blood ADAM 8 was correlated with N-stage (Spearman's correlation, γs,=0.320, p=0.011), but not correlated with T-stage or M-stage. Pearson's correlations showed blood ADAM 8 was closely correlated with pre-inflammatory cytokines (IL-23, SDF-1α/CXCL12, all of p<0.05), but not correlated with pro-angiogenic cytokines (IL-8, sCD40L). ROC curve and logistic regression demonstrated serum ADAM 8 showed higher diagnostic accuracy (sensitivity, 73.7%; specificity, 86.2%) than CEA (sensitivity, 23.1%; specificity, 91.4%). Combination of ADAM 8 and CEA more increased the diagnostic accuracy to predict GC (sensitivity, 81.8%; specificity, 84.0%). Conclusion: Blood ADAM 8 is a promising biomarker for early detection of GC. Citation Format: Jae-Il Choi, Joon Seong Park, Hye Won Chung, Jong-Baeck Lim. A disintegrin and metalloproteinase 8 as a potential blood biomarker for early diagnosis of gastric cancer [abstract]. In: Proceedings of the Annual Meeting of the American Association for Cancer Research 2020; 2020 Apr 27-28 and Jun 22-24. Philadelphia (PA): AACR; Cancer Res 2020;80(16 Suppl):Abstract nr 739.