Abstract 5747: Microwave responsive thermosensitive lipid nanoparticles for spatiotemporal delivery of chemotherapeutics

Abstract

Abstract Pancreatic cancer is a highly lethal malignancy with an urge for the exploration of novel treatments. One promising approach is microwaved-induced hyperthermia, which has shown great antitumor and synergistic effects when combined with other therapies. The application of microwaves produces heat by dielectric hysteresis which depends on the dielectric properties of molecules. Ionic liquids (ILs) are compounds composed of ions with <100 °C melting point and have strong dielectric properties. In short, we propose the creation of microwave-sensitive nanoparticles using IL, enabling precise hyperthermia induction with low-powered microwaves in localized areas. To achieve this, we stabilize a core composed of 1-butyl-methylimidazolium bromide or 1-butyl-3-methylimidazolium hexafluorophosphate using Span 80, Tween 20, and Triton-X as surfactants, along with phosphatidylcholine lipids as “cosurfactants”. Employing a double emulsion procedure for fabrication, nanoparticles are characterized using dynamic light scattering, Fourier-transform infrared spectroscopy, and transmission electron microscopy. Furthermore, we assess microwave sensitivity by comparing thermal changes between liposomes loaded with 120 mM and 250 mM NaCl solutions. Subsequently, we evaluate biocompatibility and heat effects by incubating IL nanoparticles with BXPC3 and KPC cells, monitoring cell viability through MTT assays. We aim to confine the ILs within the nanoparticle core rather than incorporating them into the membrane. We anticipate increased microwave sensitivity, translating to reduced off-target effects and enhanced precision in hyperthermia treatment. This research holds promise for improving the therapeutic outcomes of pancreatic cancer treatment. Citation Format: Cesar B. Aparicio-Lopez. Microwave responsive thermosensitive lipid nanoparticles for spatiotemporal delivery of chemotherapeutics [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 5747.