Abstract 5642: Investigation of effective combination of anti-cancer drugs and radiation therapy against non-small cell lung cancer using NCI-H520 cell line

Abstract

Abstract Background: Radiotherapy (RT) is used for to treat unresectable locally advanced non-small cell lung cancer (NSCLC). For better disease control, RT has recently been combined with chemotherapy (CTx) in a variety of ways. This study examined the most effective combination methods of CTX and RT against NSCLC. Material and Methods: Human lung cancer cell line (NCI-H520) and various chemotherapeutic agents (paclitaxel, docetaxel, gemcitabine, and cisplatin) were used in this study. The radiation doses used were 0, 2, 4 and 8 Gy. After processing of various combinations according to the radiation dose and concentrations of chemotherapeutic agents, the level of cell survival was quantified by a MTT assay. The combination index was calculated using the Chou and Talalay method on Calcusyn software and used to examine the synergism between CTx and RT. Results: Among the various combinations of chemotherapeutic agents and radiation doses, concurrent chemoradiation therapy (CCRT) led to the highest rate of apoptosis and synergism. CTx followed by RT was the most effective combination when taxane was administrated. When high-dose chemotherapeutic agents were added to CCRT, induction CTx resulted in higher rate of apoptosis rate and synergism than consolidation CTx. Conclusions: CCRT is the most effective combination when RT is combined with CTx in the NCI-H520 cell line. Induction CTx is more effective than consolidation CTx if a high-dose chemotherapeutic agent is added to CCRT. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 5642.