Abstract 5535: Availability of a newly designed technique, triple-combination array analysis: Identification of estrogen receptor 1 gene in hepatocellular carcinoma using this method

Abstract

Abstract Background: Hepatocellular carcinoma (HCC) is one of the top five causes of cancer-related deaths worldwide. Recent developments in the treatment of HCC have failed to treat completely or prevent HCC. Genome-wide gene-expression profile analyses can now detect many candidate genes that are modified by HCC. We have developed a new technique to identify suppressor genes of HCC: triple-combination array analysis. Methods: Triple-combination array analysis, which combines gene expression profiles, single nucleotide polymorphism arrays and methylation arrays, was performed on each HCC tissue sample. Subsequently, samples from 48 HCC patients were subjected to quantitative real-time reverse-transcriptase polymerase chain reaction (PCR) and methylation-specific PCR. Results: Using this method, estrogen receptor 1 (ESR1) was detected as a candidate suppressor gene. Of the 48 clinical samples, 40 (83.3%) showed ESR1 promoter hypermethylation. In 24 (50%) HCC samples, the expression level of ESR1 gene was decreased by >90%, which was significantly related to hepatitis B virus, high liver damage score, and pathological invasion of the intrahepatic portal vein. Conclusions: The present study indicates that triple-combination array is an available and convenient technique for detecting the genes with altered expression in disease, and we suggest that the ESR1 gene acts as a suppressor gene in HCC. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 5535. doi:1538-7445.AM2012-5535