Abstract
Abstract In this study, CD44+ HCT-116 cells were considered as colon cancer stem-like cells (CSCs) and CD44− HCT-116 cells were considered colon non-cancer stem cells (NCSCs). CD44+ cells were isolated by magnetic bead sorting. CD44+ cells outgrew CD44− cells in vitro. GFP-CD44+ cells preferentially migrated from the center of the spheroids after co-culture with RFP-CD44− cells. GFP-CD44+ cells grew to a greater extent subcutaneously in nude mice, when compared to the CD44− cells. Equal numbers of GFP CSCs and the RFP NCSCs were mixed and co-injected in the spleen of nude mice where the cells metastasized to the liver. GFP CSCs were found in greater numbers compared to RFP NCSCs in the liver of nude mouse. GFP CSCs and RFP NCSCs were co-injected in the wall of the cecum. GFP CSCs were observed in metastases formed in the small intestine and colon wall, to a much greater extent than RFP-NCSC. Simultaneous color-coded imaging of CSCs and NCSCs in the present study has demonstrated two very different subsets of cells within the HCT-116 cell line with respect to tumor growth and metastasis. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 5208. doi:10.1158/1538-7445.AM2011-5208
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