Abstract 5131: Differentially expressed proteins in esophageal squamous cell carcinoma tissue

Abstract

Abstract Purpose: To identify differentially expressed proteins among the esophageal squamous cell carcinoma(ESCC) carcinoma tissue, adjacent normal tissue, normal esophageal mucosa, in order to look out the protein related to different stages and study the molecular mechanism and occurrence of the esophageal squamous cell carcinoma. Methods: Tissue specimens of ESCC and the corresponding clinical data database of ESCC patients were established, a total of 69 patients were selected according to distribution of their sex and age. Two-dimensional gel electrophoresis technology to electrophoresis and get the separation protein of cancerous tissues, adjacent tissues and normal esophageal tissues,then ImageMaster 2D Platinum software was used to search, quantify, match and statistical analysise the proteins on the gel image. 4700 Proteomics Analyzer (TOF/TOFTM) was used to analyze protein spots, in which differentially expressed proteins meet standards were further searched in the Matrix Science, London, UK protein database. Results: Totle 44 and 29 kinds of differentially expressed proteins were screened from esophageal cancer tissues, adjacent noncancerous tissues and normal tissues, among which, there were 13 differentially expressed proteins between esophageal cancer tissues and adjacent noncancerous tissues, 14 differentially expressed proteins between esophageal cancer tissues and normal tissues, 17 between esophageal adjacent noncancerous tissues and normal tissues. These differential expression proteins mainly involved in PKC-catalyzed phosphorylation of inhibitory phosphoprotein of myosin phosphatase, nuclear factor of activated T cells(NFAT) pathway, Integrin Signaling Pathway, Cell to Cell Adhesion Signaling, Calmodulin(CaM) nuclear factor of activated T cells, Mineral absorption of calcium binding protein path way(S100), Path way of vascular smooth muscle contraction affe cted by caldesmon, Apoptotic Signaling in Response to DNA Damage path way, which had the function of cytoskeleton structure, protein phosphorylation, acetylation of aminoend, regulation of calcium combination,tumor cell movement, signal transduction, cell differentiation, cell apoptosis, muscle contraction, translation regulation, gene expression, energy metabolism, cell proliferation. Conclusion: This study study achieved differentially expressed protein in ESCC tissue patients, provides new basis for the study of esophageal cancer carcinogenesis mechanism. Citation Format: Fuchun Si. Differentially expressed proteins in esophageal squamous cell carcinoma tissue [abstract]. In: Proceedings of the Annual Meeting of the American Association for Cancer Research 2020; 2020 Apr 27-28 and Jun 22-24. Philadelphia (PA): AACR; Cancer Res 2020;80(16 Suppl):Abstract nr 5131.