Abstract 5111: Protective effect of Traditional Chinese Medicine formula LQ against cyclophosphamide-induced renal damage in nude mice.

Abstract

Abstract TCM formula LQ is an herbal cocktail. Cyclophosphamide (CYC) is widely used in cancer chemotherapy. However renal damage is a dose-limiting side effects of CYC. In the current study, we determined if LQ can protect against damage. The heart, liver, spleen, lung, kidney, and intestine of a mouse from control, LQ pretreatment, CYC pretreatment, and CYC+LQ treatment were stained with HE. The histology of each organ was observed under microscopy. The histology of the heart, liver, spleen, lung and intestine showed normal histopathological structure in all groups. The kidneys in the control mice, LQ pretreated mice, and CYC+LQ pretreated mice showed normal architecture of kidney cortex and medulla. The cortex showed normal renal corpuscle, proximal convoluted tubules, and distal convoluted tubules. The inner medulla and outer medulla all contained a thick ascending limb of the loop of Henle, and interstitial connective tissue. However, kidney of the CYC pretreatment mice showed damage in structures compare with other groups. The toxic effect of CYC on the kidney involved the glomeruli, the tubules, and the interstitium. The structures of the cortex in CYC pretreated group showed disconnection between glomeruli and tubules. The ducts in the medulla were destroyed. LQ also protected against weight loss and cachexia induced by CYC. LQ may be a promising drug for combination with CYC to improve its therapeutic index. Citation Format: Lei Zhang, Chengyu Wu, Robert M. Hoffman. Protective effect of Traditional Chinese Medicine formula LQ against cyclophosphamide-induced renal damage in nude mice. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 5111. doi:10.1158/1538-7445.AM2013-5111