Abstract 4570: Decreased interleukin-17RA expression inhibits the tumorigenesis and independently predicts good prognosis of colorectal cancer patients

Abstract

Abstract Background: Interleukin-17 receptor type A (IL-17RA) plays critical role in promoting early colorectal tumor development and affects immune response in tumorigenesis. Our previous study have reported interleukin-17A modulated tumorigenesis and affected metastasis in colorectal cancers, but the roles of its receptor (IL-17R) in colorectal cancers remain unknown. This study explores potential role and function of IL-17RA in colorectal cancers. Materials and Methods The expression of IL-17RA was determined in colorectal cancer tissues and adjacent normal tissues using quantitative real-time PCR and immunohistochemistry. The IL-17RA expression level and clinical parameters were then analyzed. To investigate the functional significance of IL-17RA, IL-17RA knockdown cells were analyzed using transwell assay in vitro and implanted subcutaneously in mice, and monitored for primary tumor growth and angiogenesis. Results IL-17RA was overexpression in colorectal cancer tissues compared with adjacent normal tissues (P<0.05).The elevated expression level of IL-17RA was associated with poor survival (P<0.001). In vitro, the IL17RA knockdown cells reduced migration and invasion. In mice, the decreased IL-17RA inhibited tumor growth and angiogenesis. Conclusions These results demonstrated that IL-17RA plays a critical role in growth, angiogenesis and prognosis of colorectal cancer. Citation Format: CHIH-YUNG YANG, Jeng-Kai Jiang. Decreased interleukin-17RA expression inhibits the tumorigenesis and independently predicts good prognosis of colorectal cancer patients. [abstract]. In: Proceedings of the 107th Annual Meeting of the American Association for Cancer Research; 2016 Apr 16-20; New Orleans, LA. Philadelphia (PA): AACR; Cancer Res 2016;76(14 Suppl):Abstract nr 4570.