Abstract 4304: Development of a new nucleoside triphosphate transporter (NTP) technology for rapid DNA labeling that measures cell cycle progression in live cancer cells

Abstract

Abstract At its core, cancer is a disease of uncontrolled cell division. Mutations in different genes cause or accelerate cancer by increasing cell division rates or inhibiting cell proliferation control mechanisms such as apoptosis and tumor suppression. Traditional DNA incorporation cell proliferation assays that label nucleic acids (BrdU, EdU) have been popular but are limited by requiring cell fixation, high cost and lengthy protocols. Here, we have developed a new NTP transporter technology that allows the incorporation of Cyanine-3 fluorescently labeled dUTP's into DNA during the S-phase of the cell cycle to measure cell proliferation in live cancer cells (U2OS). Similar cell cycle profiles were produced using this new NTP transporter technology as analyzed by FACS analysis and live cell imaging vs. BrdU based cell proliferation assays. This technology will allow new insights into cancer cell proliferation and progression. Citation Format: Nick Asbrock, Vi Chu, Kevin Su, Tomas Kraus. Development of a new nucleoside triphosphate transporter (NTP) technology for rapid DNA labeling that measures cell cycle progression in live cancer cells [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2018; 2018 Apr 14-18; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2018;78(13 Suppl):Abstract nr 4304.